![]() It can be used to process and analyze images. ImageJ is a free and open source image processing program. The main purpose of FiFi is to provide a full-featured imageJ2 distribution with a wide range of plugins bundled. ![]() This image processing package is based on ImageJ2 and is available as a free open source download. Unless otherwise specified in a credit line, all third-party material in this chapter is subject to the chapter’s Creative Commons license. You are permitted to use, distribute, adapt, and reproduce this chapter in any medium or format under the Creative Commons Attribution-NonCommercial-nc 2.5 International License ( ). It should be noted that many seemingly analog controls such as focus knobs, magnification knobs, and stigmators are actually digital interfaces mounted on hand-panel controllers that connect to a microscope control computer via a USB cable. The use of a graphical user interface (GUI) on most modern microscopes is required for the operator to control the hardware. ImageJ and Fiji Abstract Software are essential for scanning electron microscopists and X-ray microanalysts. If you need a more user-friendly package with specific features for the analysis of biological images, Fiji is a better choice. If you need a comprehensive image processing and analysis package, ImageJ is a good choice. ![]() ![]() So, which software is better? That depends on your needs. Fiji, on the other hand, is less comprehensive but has a number of advantages over ImageJ, including: a more user-friendly interface better support for multithreading and a number of features specifically designed for the analysis of biological images. ImageJ has a wide range of features, including: support for a wide range of image formats an integrated macro language plugin support and a comprehensive set of image processing and analysis functions. Fiji is a free and open source image processing software developed by the Fiji team. Below is a montage of a time series, but you could just as easily use this method to display a z-stack.ImageJ is a free and open source image processing software developed by the National Institutes of Health. This post has been dealing with multi channel data but there are plenty of circumstances where you may want to use montages to display other multi dimensional data. If you’d prefer a single row, you can always specify the dimensions as 5 columns x 1 row which looks like this: If you’ve chosen the default layout and checked the “Label Slices” box you’ll have a 3×2 grid with one blank frame (I like putting a border of 2-5 pixels between images):
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